Effect of Helium-neon Laser Irradiation on Antitumor Activity of Lak and Il-2 in Mice with Lewis Lung Carcinoma

Among successful approaches to cancer immunotherapy, the combination of cytokines application and adoptive transfer of lymphoid effector cells represents the most promising one. To stimulate functional activity of lymphoid cells used for adoptive transfer, some physical factors may be used [1, 2]. It has been shown [1, 3, 4] that the low-energy laser irradiation possesses immunomodulating properties. Laser irradiation in the red spectral area influences the proliferative activity of peripheral blood lymphocytes [5]. Laser irradiation has the protective effect on lymphoid cells [6] and stimulates cytokine production by lymphocytes [7–10]. Earlier we have shown that He-Ne laser irradiation stimulates functional activity of innate and adaptive immunity effector cells both in vitro and in vivo [11]. The present work continues the study of potential immunomodulating action of laser irradiation and is aimed on the research of efficacy of the low-energy laser irradiation for treatment of Lewis lung carcinoma (LLC) by adoptive immunotherapy in combination with IL-2 administration. 50 male C57/black mice, 12–16 weeks old weighing 20–25 g were obtained from the vivarium of R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology NAS of Ukraine (Kyiv, Ukraine). All animal procedures were carried out according to the rules of Ethic Committee. LLC cells were routinely passed by s.c. transplantation (3 · 10 cells in 0.02 ml PBS per animal). The animals were housed in 5 groups: 1) 1st group (control) — no treatment; 2) 2nd group — animals received adoptive immunotherapy; 3) 3rd group — the animals were treated with LAK irradiated in vitro with HeNe laser; 4) 4 group received adoptive immunotherapy in a combination with laser irradiation of blood — LEB; 5) in 5th group animals were treated with LAK irradiated in vitro and LEB. The dynamics of tumor growth was estimated by tumor volume and weight, and metastasis number per animal was evaluated as well. The mouse spleens were removed aseptically and mechanically homohenized, splenocytes were washed,